Interaction between Xanthoxylin and Bovine Serum Albumin

The interaction between xanthoxylin (XT) and bovine serum albumin (BSA) under simulative physiological conditions has been analyzed in detail by various spectroscopic techniques including fluorescence, circular dichro- ism (CD), and Fourier transform infrared (FT-IR) spectroscopy. Fluorescence quenching data revealed that the quenching constants (K) were 3.31×105, 2.03×105 and 0.94×105 L·mol-1 at 286, 298 and 310 K, respectively. Based on the fluorescence results, the fluorescence quenching mechanism of the interaction between XT and BSA has been found to be combined static and dynamic quenching. Thermodynamic parameters △H0, △S0 and △G0 sug- gested that the hydrophobic force played a main role in binding of XT to BSA. The effect of XT on the conforma- tion of BSA was analyzed by FT-IR spectroscopy and quantitatively calculated from CD spectroscopy with reduc- tion of α-helical content by about 3.9%. In addition, the effect of common ions on the binding constant was also dis- cussed.

作 者： WEN Maogui TIAN Jianniao HUANG Yonglin BIAN Hedong CHEN Zhengfeng LIANG Hong   作者單位： WEN Maogui,TIAN Jianniao,CHEN Zhengfeng,LIANG Hong(Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University),Ministry of Education of China, Guilin, Guangxi 54904, China)

HUANG Yonglin(Guangxi Institute of Botany,Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences,Guilin,Guangxi 541004,China)

BIAN Hedong(Chemistry and Chemical Engineering,Guangxi Normal University,Guilin,Guangxi 541004,China) 

刊 名： 中國化學（英文版）  SCI 英文刊名： CHINESE JOURNAL OF CHEMISTRY  年，卷(期)： 2009 27(2)  分類號： O6  關鍵詞： bovine serum albumin   xanthoxylin   Fourier transform infrared spectra (FT-IR)   fluorescence quench-ing  

【Interaction between Xanthoxylin and 】相關文章：

Between the Sea and the Sky05-04

Gap between Men and Women05-04

Between Friends 朋友之間05-04

Physicochemical interaction and its influence on deep bed filtration process04-26

Studies on the Interaction of Beryllon Ш with Proteins by Voltammetric Technique and its Application04-27

Experimental study on the interaction of fine water mist with solid pool fires04-27

Preliminary study on interaction of water mist with diffusion flame of liquid fuels04-27

Difference between man and woman? 男女之不同05-04

Comparison between controlled landfill reactor and conditioned landfill bioreactor04-25

EFFECTS OF ENSO ON THE RELATIONSHIP BETWEEN IOD AND SUMMER RAINFALL IN CHINA04-27