Expression of PHGPx in mammalian cells and its antiviral effect against coxsackievirus group B

To express human phospholipid hydroperoxide glutathione peroxidase (PHGPx) in eukaryotic cells and to study its antiviral effect against Coxsackievirus B3m (CVB3m) in vitro, PHGPx cDNA was amplified from a human testis library using specific primers and cloned into expression vector pcDNA3.1/His. Expression of PHGPx was performed in COS-1 cells. The antiviral effect was studied by the treatment of HeLa cells with the recombinant PHGPx. Results showed that the activity of PHGPx expressed in COS-1 cells was 5-fold higher than that in control group, and it inhibited the cytopathic effect on HeLa cells caused by CVB3m. It can be concluded that recombinant PHGPx expressed in COS-1 ceils has antiviral effect against CVB3m in vitro.

作 者： ZHAO Wenran ZHONG Xuekuan ZHOU Lingwang   作者單位： Institute of Keshan Disease, Chinese Research Center for Endemic Disease Control, Harbin Medical University, Harbin 150086, China  刊 名： 自然科學進展（英文版）  SCI 英文刊名： PROGRESS IN NATURAL SCIENCE  年，卷(期)： 2005 15(7)  分類號： Q5  關鍵詞： PHGPx   gene expression   coxsackievirus group B   antiviral effect  

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